Journal: PLoS ONE
Article Title: Acyl Chain-Dependent Effect of Lysophosphatidylcholine on Endothelium-Dependent Vasorelaxation
doi: 10.1371/journal.pone.0065155
Figure Lengend Snippet: The rings were incubated in 200 µl aerated PSS under cell culture conditions at 37°C for 1 h. Thereafter, buffer was replaced with fresh PSS supplemented with PSS (control) or 10 µM LPC followed by further incubation under cell culture conditions at 37°C for 1 h. (A) 6-keto PGF 1α , a stable degradation product of PGI 2 (B), TXB 2 , a stable degradation product of TXA 2 , (C) PGE 2 and (D) PGF 2α were quantified by EIA assays and rings were solubilized for protein quantification. shown in A and B are means ± SD of four experiments and those in C and D of three experiments, done in triplicates. *P<0.05, **P<0.01***P<0.001.
Article Snippet: The concentrations of 6-Keto-PGF 1α , TXB 2 , PGE 2 and PGF 2α were measured by corresponding correlate-EIA kits (Cayman, Ann Arbor, MI) according to the manufacturer's protocol.
Techniques: Incubation, Cell Culture